antimmp 9  (AMS Biotechnology)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Relationship among LRP1 expression, Pyk2 phosphorylation and MMP‐9 activation in left ventricular remodelling after myocardial infarction

doi: 10.1111/jcmm.13113

Figure Lengend Snippet: Temporal and spatial evolution of myocardial MMP‐9 and MMP‐2 activation and expression after MI. ( A ) Representative zymography analysis results showing myocardial MMP‐9 and MMP‐2 activity. Bar graphs showing the mean ± S.D. of MMP‐9 and MMP‐2 activity in the remote, peri‐infarct and infarct zones at 1 ( B and E ), 10 ( C and F ) and 21 ( D and G ) days after MI. ( H–M ) Real‐time PCR showing MMP‐9 and MMP‐2 mRNA expression levels. Data were processed using a specially designed software program based on the Ct values of each sample and normalized to 18s rRNA, which served as an endogenous control. The results are shown as the mean ± S.D. of MMP‐9 and MMP‐2 mRNA expression levels in the remote, peri‐infarct and infarct zones at 1 ( H and K ), 10 ( I and L ) and 21 ( J and M ) days after MI. n = 8. * P < 0.05 versus remote; ** P < 0.01 versus remote; ## P < 0.01 versus peri‐infarct; *** P < 0.005 versus remote; ### P < 0.005 versus peri‐infarct.

Article Snippet: MMP‐9 , TP221 , AMS Biotechnology.

Techniques: Activation Assay, Expressing, Zymography, Activity Assay, Real-time Polymerase Chain Reaction, Software

Journal: Journal of Cellular and Molecular Medicine

Article Title: Relationship among LRP1 expression, Pyk2 phosphorylation and MMP‐9 activation in left ventricular remodelling after myocardial infarction

doi: 10.1111/jcmm.13113

Figure Lengend Snippet: Representative confocal microscopy images showing LRP1, MMP‐9 and pERK1,2 expression levels in the remote, peri‐infarct and infarct zones after MI. ( A ) Immunostaining of heart cross sections showing the temporal evolution of pERK1,2 (grey), MMP‐9 (green) and LRP1 (red) expression levels after MI. ( B ) Immunostaining of heart cross sections showing the temporal evolution of pERK1,2 (grey), MMP‐2 (green) and LRP1 (red) expression levels after MI. Cell nuclei were counterstained with DAPI (blue). Scale bars, 50 μm.

Article Snippet: MMP‐9 , TP221 , AMS Biotechnology.

Techniques: Confocal Microscopy, Expressing, Immunostaining

Journal: Journal of Cellular and Molecular Medicine

Article Title: Relationship among LRP1 expression, Pyk2 phosphorylation and MMP‐9 activation in left ventricular remodelling after myocardial infarction

doi: 10.1111/jcmm.13113

Figure Lengend Snippet: Representative confocal microscopy images showing LRP1, MMP‐9 and pPyk2 levels in the remote, peri‐infarct and infarct zones after MI. Immunostaining of heart cross sections showing the temporal evolution of pPyk2, MMP‐9 and LRP1 expression levels after MI. LRP1 is shown in red; MMP‐9, in green; and pPyk2, in grey. A and B inserts are amplified in A’ and B’, respectively, to show colocalization of the three markers in light yellow. Cell nuclei were counterstained with DAPI (blue). Scale bars, 50 μm.

Article Snippet: MMP‐9 , TP221 , AMS Biotechnology.

Techniques: Confocal Microscopy, Immunostaining, Expressing, Amplification

Journal: Journal of Cellular and Molecular Medicine

Article Title: Relationship among LRP1 expression, Pyk2 phosphorylation and MMP‐9 activation in left ventricular remodelling after myocardial infarction

doi: 10.1111/jcmm.13113

Figure Lengend Snippet: Comparison of the hypoxia impact on LRP1, pPyk2 levels and MMP‐9/MMP‐2 activation in macrophages and fibroblasts. Quiescent macrophages and fibroblasts were exposed to normoxic or hypoxic conditions for 18 hrs. Representative Western blot analysis showing LRP1, pPyk2, total Pyk2 and β‐tubulin bands ( A & G ). Bar graphs showing the mean ± S.D. of LRP1 normalized to β‐tubulin levels ( B & H ) and pPyk2/total Pyk2 ratio ( C & I ). ( D & J ) Representative zymography analysis showing MMP‐9 and MMP‐2 activity and bar graphs showing the mean ± S.D. of MMP‐9 ( E & K ) and MMP‐2 ( F & L ) activity levels. Results are shown as the mean ± S.E.M. of three independent experiments performed in triplicate. *** P < 0.001 versus normoxia. NO: normoxia; HO: hypoxia.

Article Snippet: MMP‐9 , TP221 , AMS Biotechnology.

Techniques: Activation Assay, Western Blot, Zymography, Activity Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Relationship among LRP1 expression, Pyk2 phosphorylation and MMP‐9 activation in left ventricular remodelling after myocardial infarction

doi: 10.1111/jcmm.13113

Figure Lengend Snippet: Comparison of the hypoxia effect on pPyk2 levels and MMP‐9/MMP‐2 activation in control ( Lrp1 +/+ ) and LRP1‐deficient fibroblasts ( Lrp1 −/− ). Quiescent Lrp1 +/+ and Lrp1 −/− fibroblasts were exposed to normoxic or hypoxic conditions for 18 hrs. ( A ) Representative Western blot analysis showing LRP1, pPyk2, total Pyk2 and β‐tubulin levels and bar graphs showing the mean ± S.D. of LRP1 levels normalized to β‐tubulin ( B ) and pPyk2/total Pyk2 ratio ( C ). ( D ) Representative zymography analysis showing MMP‐9 and MMP‐2 activity levels and bar graphs showing the mean ± S.D. of MMP‐9 ( E ) and MMP‐2 activity levels ( F ). Results are shown as the mean ± S.E.M. of three independent experiments performed in triplicate. *** P < 0.001 versus normoxia. ( G ) Representative scheme showing the crucial role of LRP1 up‐regulation in hypoxic cardiac fibroblasts in cardiac remodelling.

Article Snippet: MMP‐9 , TP221 , AMS Biotechnology.

Techniques: Activation Assay, Western Blot, Zymography, Activity Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Relationship among LRP1 expression, Pyk2 phosphorylation and MMP‐9 activation in left ventricular remodelling after myocardial infarction

doi: 10.1111/jcmm.13113

Figure Lengend Snippet: Assays‐on‐demand used for real‐time PCR analysis

Article Snippet: MMP‐9 , TP221 , AMS Biotechnology.

Techniques: Real-time Polymerase Chain Reaction

Journal: Journal of Cellular and Molecular Medicine

Article Title: Relationship among LRP1 expression, Pyk2 phosphorylation and MMP‐9 activation in left ventricular remodelling after myocardial infarction

doi: 10.1111/jcmm.13113

Figure Lengend Snippet: Primary antibodies used for immunohistochemical analysis

Article Snippet: MMP‐9 , TP221 , AMS Biotechnology.

Techniques: Immunohistochemical staining

Journal: Journal of Cellular and Molecular Medicine

Article Title: Relationship among LRP1 expression, Pyk2 phosphorylation and MMP‐9 activation in left ventricular remodelling after myocardial infarction

doi: 10.1111/jcmm.13113

Figure Lengend Snippet: Primary antibodies used for immunohistochemical analysis

Article Snippet: MMP‐9 , TP221 , AMS Biotechnology.

Techniques: Immunohistochemical staining